Physics Reports vol.90

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L. , 1997. 16 Practical High-Performance Liquid Chromatography General textbooks on chromatography: E. , 2004. M. , 2009. F. Poole, The Essence of Chromatography, Elsevier, Amsterdam, 2002. K. E. R. Haddad, Principles and Practice of Modern Chromatographic Methods, Academic, London, San Diego, 1995. 1 THE CHROMATOGRAPHIC PROCESS Definition Chromatography is a separation process in which the sample mixture is distributed between two phases in the chromatographic bed (column or plane). One phase is stationary whilst the other passes through the chromatographic bed.

2010. R. W. Dolan, High-Performance Gradient Elution, WileyInterscience, Hoboken, 2007. R. J. L. , 1997. 16 Practical High-Performance Liquid Chromatography General textbooks on chromatography: E. , 2004. M. , 2009. F. Poole, The Essence of Chromatography, Elsevier, Amsterdam, 2002. K. E. R. Haddad, Principles and Practice of Modern Chromatographic Methods, Academic, London, San Diego, 1995. 1 THE CHROMATOGRAPHIC PROCESS Definition Chromatography is a separation process in which the sample mixture is distributed between two phases in the chromatographic bed (column or plane).

23). The peaks become wider and asymmetric and the retention time changes at the same time. Tailing is accompanied by decreased retention and fronting by an increase in k. By definition, the column has not quite reached the overload level if the change in the k value is less than 10% with respect to infinitely small amounts of sample. Most phases are not overloaded if the sample mass is less than 10 mg per gram of stationary phase. 0 g, depending on the column dimensions). 23 Mass overload. Top: 2 mg each of acetophenone (first peak) and veratrole; bottom: 2 mg each.

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