By Gary D. Christian
Broadly revised and up-to-date with a extra smooth style and a brand new, two-color layout, this 6th variation offers with ideas and methods of quantitative research. Examples of analytical ideas are drawn from such components as lifestyles sciences, scientific chemistry, air and water pollutants, and commercial analyses. New to this variation: Excel spreadsheets on CD-ROM * New chapters on reliable laboratory perform, in addition to genomics and proteomics * A extra smooth taste.
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Chelidonine) and blue-violet zones are found. ). 8. 5:10) A UV-254nm (without chemical treatment) B UV-365nm (without chemical treatment) C Dragendorff reagent/NaNO, (DRG No. 13 13) -+ vis. Fig. 20A Colchici semen (1,2). 151T2) is found in the methanolic extract (2) only. 85 and two zones at the solvent front are detected in UV-365 nm. 25 (TI) minor allcaloids such as colchiceine, N-acetyl demecolcine and 1-ethyl-2-demethyl colchiceine also show a yellow-white fluorescence, while 0-benzoyl colchiceine, Nformyl-deacetyl colcl~iceineand N-methyl demecolcine fluoresce blue.
G. g. 7. Fig. 55 (phenol carboxylic acids, sanguinarine, protoberberines) in sample 2. - E With DRG reagent two main and one minor brown alkaloid zone (vis) are detectable in sample 3. 6 and allocryptopine in the lower R, range. In the methanolic extract (2) these alkaloids are present in low concentration only. g. g. g. rutin, quercetin-3,7-diglucosido-3-arabinosjde) and the aglycones at the solvent front. - - - - 1 Allialoid Drugs 37 Pig. 15 Fig. 6) b isochloro- Fig. 5:5) + alkaloids A Natural products-polyethylene glycol reagent (NPIPG No.
Gelsemii radix Drug sample 1 Gelsemii radix, (alkaloid extraction method B, 40yl) Reference T1 sempervirine colllyou~ld T2 gelsernine T3 isogelsemine Solverit system Detection Fig. 8A Fig. 8 aetone-light petroleum-diethylamine (20:70:10) A UV-365nm (without chemical treatment) 0 Dragendorff reagent (DRG No. 35) does not fluoresce. ). - - I me- Alkaloid Drugs 29 Harmalae semen Drug sample Reference compound 1 Harmalae semen, (methanol extract, 3 0 ~ 1 ) 'rl harinalol T2 harmaline T3 harmane T4 harmine T5 harm01 Solve~rtsyste~rr Fig.