Analytical Biotechnology. Capillary Electrophoresis and by Csaba Horvath, John G. Nikelly

By Csaba Horvath, John G. Nikelly

content material: High-resolution nanotechnique for separation, characterization, and quantitation of micro- and macromolecules : capillary electrophoresis / Noberto A. Guzman, Luis Hernandez, and Shigeru Terabe --
purposes of capillary quarter electrophoresis to quality controls / R.G. Nielsen and E.C. Rickard --
research of cyclic nucleotides by means of capillary electrophoresis utilizing ultraviolet detection / Luis Hernandez, Bartley G. Hoebel, and Noberto A. Guzman --
On-column radioisotope detection for capillary electrophoresis / Stephen L. Pentoney, Jr., Richard N. Zare, and Jeff F. Quint --
ideas for an analytical exam of organic prescribed drugs / Eleanor Canova-Davis, Glen M. Teshima, T. Jeremey Kessler, Paul-Jane Lee, Andrew W. Guzzetta, and William S. Hancock --
Analytical chemistry of healing proteins / R.M. Riggin and N.A. Farid --
development and experimental validation of protein impurity immunoassays for recombinant DNA items / Vincent Anicetti --
minimal variance purity regulate of preparative chromatography with simultaneous optimization of yield : an online species-specific detector / Douglas D. Frey --
Amino acid sequence-mass spectrometric analyses of mating pheromones of the ciliate Euplotes raikovi / R.A. Bradshaw, S. Raffioni, P. Luporini, B. Chait, T. Lee, and J. Shively --
Micropellicular sorbents for quick reversed-phase chromatography of proteins and peptides / Krishna Kalghatgi and Csaba Horváth --
Displacement chromatographic separations on [beta]-cyclodextrin-silica columns / Gyula Vigh, Gilberto Quintero, and Gyula Farkas.

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F i g u r e 2 shows the c o m p a r i s i o n between d i g e s t s of hGH and [desamido-Asn-149] hGH R e s i d u e Asn-149 i s l o c a t e d i n fragment 15 of the t r y p t i p o s i t i o n 146 to 158 t h a His-Asn-Asp-Asp-Ala-Leu-Leu-Lys-COOH. I t i s apparent t h a t fragment 15 of the desamido d e r i v a t i v e has s h i f t e d to a l o n g e r m i g r a t i o n time, c o n s i s t e n t w i t h the f o r m a t i o n of an a d d i t i o n a l n e g a t i v e charge from the c o n v e r s i o n of the a s p a r a g i n e amide t o an a s p a r t i c a c i d carboxylate.

M o b i l i t y i n the open t u b u l a r mode i s p r e d o m i n a n t l y r e l a t e d t o c h a r g e , shape, and s i z e o f the a n a l y t e as w e l l as t o the p r o p e r t i e s of the e l u t i n g s o l v e n t ( 9 ) . Thus, the s e p a r a t i o n o f i m p u r i t i e s and d e g r a d a t i o n p r o d u c t s t h a t have s i m i l a r shapes and s i z e s w i l l be d e t e r m i n e d p r i n c i p a l l y by d i f f e r e n c e s i n t h e i r n e t charge ( 2 0 ) . Adjustment o f pH t o a v a l u e near the m i d p o i n t o f the i s o e l e c t r i c p o i n t ( p i ) range of the a n a l y t e s w i l l t e n d t o maximize t h e i r n e t charge d i f f e r e n c e s , p r o v i d e d t h a t the p r o t e i n s a r e s o l u b l e near t h e i r p i .

Sittampalam 1989, 61, 1186-1194. 17. Nielsen, R. ; Riggin, R. ; Rickard, E. C. J. , 1989, 480, 393-401. 18. Nielsen, R. ; Rickard, E. C. J. , submitted. 19. Moring, S. ; Colburn, J. ; Grossman, P. ; Lauer, Η. H. LC-GC 1990, 8, 34-46. 20. Grossman, P. ; Wilson, K. ; Lauer, Η. H. Anal. Biochem. 1988, 173, 265-270. 21. Rose, D. ; Jorgenson, J. W. Anal. Chem. 1988, 60, 642-648. 22. Rose, D. ; Jorgenson, J. W. J. Chromatogr. 1988, 447, 117-131. 23. ; Dovichi, N. J. Science 1988, 242, 562-564. 24. Pentoney, S.

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