Analytical and Preparative Separation Methods of by Hassan Y. Aboul-Enein

By Hassan Y. Aboul-Enein

Experiences updated examine advancements on purifying and isolation huge natural molecules. The textual content presents info on high-performance liquid chromatography and capillary electrophoresis (CE) as instruments for examining biomacromolecules and constructing new biochemical and medicinal compounds. It applies biochemical separation expertise to the examine of macromolecules corresponding to proteins, polysaccharides, nucleic acids and extra.

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Isomerization of the N-terminal peptide fragment obtained after tryptic digestion of rSmp2812 can be explained by the fact that the peptide contains a cyclic linkage that formed during digestion. Since this phenomenon was only observed in the case of rSmp2812, it is likely to be related to the original N-terminal modification that led to the shift in isoelectric point. To test the hypothesis of a cyclic linkage, both the free and blocked Nterminal peptides were subjected to deuterium exchange followed by MS.

9). After detergent removal by acetone precipitation, intact rOspA was submitted to an acidic methanol treatment in order to generate fatty acid methyl esters via transmethylation. Fatty acid methyl esters were extracted into hexane, which permitted their analy- Bischoff and Bouchon S-[2,3-Bis(palmitoyloxy)-(2RS)-propyl]-N-palmitoyl-(R)-cysteinyl ("Pam3Cys")configuration (A2, A3, A4, ... : amino acids) Figure 9 Structure of the lipidated N-terminal tryptic peptide CK of rOspA containing three palmitoyl residues.

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